Impregnatie argentica pentru reticulina

    SEAP
    ID
    DA1743787
    Data
    06 Aprilie 2011
    Valoare
    697 RON
    Stare
    Oferta acceptata
    Autoritatea contractantaLocalitate
    Oradea, Bihor
    FurnizorTipul contractului
    -
    Cod CPVDescriere:
    kit. VA RUGAM SA DATI ACCEPT DOAR DACA DETINETI PRODUSUL IN STOC.
    Achizitii
    697 RON
    Cantitate: 1
    Unitate masura: kit 100 teste
    Kit IMPREGNARE ARGENTICA (silver impregnation for reticulum)
    IMPREGNARE ARGENTICA - kit pt evidentierea reticulocitelor. Kit 100 de teste. Rezultate : fibre reticulare si nervoase (negru), tesut conjuctiv (maro), colagen (galben auriu). Method Bring the section to distilled water. Put on the section 5 drops of reagent A and 5 drops of reagent B: leave to act 5 minutes. Wash the slide in distilled water. Put on the section 10 drops of reagent C: leave to act 3 minutes Double washing in distilled water. Put on the section 10 drops of reagent D: leave to act 3 minutes. Double washing in distilled water. Put on the section 10 drops of reagent E: leave to act 3 minutes. Wash in distilled water. Put on the section 10 drops of reagent F: leave to act 5 minutes. Double washing in distilled water. Put on the section 10 drops of reagent G: leave to act 5 minutes. Wash in tap water 5 minutes. Dehydrate through ascending alcohols: clear in xylene and mount. Results Reticular and nervous fibers Connective tissue Collagen black tobacco brown gold yellow Reagents Potassium permanganate solution Acid activation buffer Oxalic acid solution Ferric ammonium sulphate solution Ammoniacal silver solution Neutral formalin solution Sodium hyposulphite fixing solution 18 ml 18 ml 30 ml 30 ml 30 ml 30 ml 30 ml