Impregnatie argentica pentru reticulina
SEAPIDStare
DA1743787
Data06 Aprilie 2011
Valoare697 RON
Oferta acceptata
Autoritatea contractantaLocalitateOradea, Bihor
FurnizorTipul contractului-
Cod CPVDescriere:kit.
VA RUGAM SA DATI ACCEPT DOAR DACA DETINETI PRODUSUL IN STOC.
Achizitii697 RON
Cantitate: 1
Unitate masura: kit 100 teste
Kit IMPREGNARE ARGENTICA (silver impregnation for reticulum)
IMPREGNARE ARGENTICA - kit pt evidentierea reticulocitelor.
Kit 100 de teste.
Rezultate : fibre reticulare si nervoase (negru), tesut conjuctiv (maro), colagen (galben auriu).
Method
Bring the section to distilled water.
Put on the section 5 drops of reagent A and 5 drops of reagent B: leave to act 5 minutes.
Wash the slide in distilled water.
Put on the section 10 drops of reagent C: leave to act 3 minutes
Double washing in distilled water.
Put on the section 10 drops of reagent D: leave to act 3 minutes.
Double washing in distilled water.
Put on the section 10 drops of reagent E: leave to act 3 minutes.
Wash in distilled water.
Put on the section 10 drops of reagent F: leave to act 5 minutes.
Double washing in distilled water.
Put on the section 10 drops of reagent G: leave to act 5 minutes.
Wash in tap water 5 minutes.
Dehydrate through ascending alcohols: clear in xylene and mount.
Results
Reticular and nervous fibers
Connective tissue
Collagen
black
tobacco brown
gold yellow
Reagents
Potassium permanganate solution
Acid activation buffer
Oxalic acid solution
Ferric ammonium sulphate solution
Ammoniacal silver solution
Neutral formalin solution
Sodium hyposulphite fixing solution
18 ml
18 ml
30 ml
30 ml
30 ml
30 ml
30 ml