Kit Giemsa pentru Helicobacter pylori
SEAPIDStare
DA1029428
Data29 Iunie 2010
Valoare2.500 RON
Oferta acceptata
Autoritatea contractantaLocalitateBucuresti, Bucuresti
FurnizorTipul contractului-
Cod CPVDescriere:Giemsa for Helicobacter pylori;um=kit-750 ml
Application
To demonstrate Helicobacter pylori bacterium on gastric biopsy sections. The qualitative and quantitative composition of staining mixture and the careful differentiation allow to identify bacteria on tidy ground.
Warning
The kit allows to prepare 15 assays using Coplin Jars of 50 ml. Each assay stains from 1 to 5 sections. Reagent can be introduced into the Coplin Jar and then recovere
Method
Deparaffinize sections and bring to distilled water.
Preparation of buffer solution.
Take out from bottle B 5 ml of solution and diluting 1:10: Utilize obtained solution for the preparation of Giemsa working-solution.
Preparation of Giemsa working-solution.
Take out 10 ml of reagent A and reach the volume of 40 ml with buffer solution previously prepared.
Put the solution in a Coplin Jar and immerse sections for 30 minutes.
Drain the slide without washing and put the section for 15 seconds in reagent C.
Repeat step 5 with reagent D and E.
Clear in xylene and mount.
Results
Helicobacter pylori
Nuclei
Cytoplasm
blue, in form of seagull's wing
blue
pink
Reagents
Giemsa solution modified
Acetate buffer solution
Differentiating reagent
Dehydrating reagent
Dehydrating reagent
150 ml
150 ml
150 ml
150 ml
150 ml
Achizitii2.500 RON
Cantitate: 6
Unitate masura: bucata
Kit Giemsa pentru Helicobacter pylori
Giemsa for Helicobacter pylori;um=kit-750 ml
Application
To demonstrate Helicobacter pylori bacterium on gastric biopsy sections. The qualitative and quantitative composition of staining mixture and the careful differentiation allow to identify bacteria on tidy ground.
Warning
The kit allows to prepare 15 assays using Coplin Jars of 50 ml. Each assay stains from 1 to 5 sections. Reagent can be introduced into the Coplin Jar and then recovere
Method
Deparaffinize sections and bring to distilled water.
Preparation of buffer solution.
Take out from bottle B 5 ml of solution and diluting 1:10: Utilize obtained solution for the preparation of Giemsa working-solution.
Preparation of Giemsa working-solution.
Take out 10 ml of reagent A and reach the volume of 40 ml with buffer solution previously prepared.
Put the solution in a Coplin Jar and immerse sections for 30 minutes.
Drain the slide without washing and put the section for 15 seconds in reagent C.
Repeat step 5 with reagent D and E.
Clear in xylene and mount.
Results
Helicobacter pylori
Nuclei
Cytoplasm
blue, in form of seagull's wing
blue
pink
Reagents
Giemsa solution modified
Acetate buffer solution
Differentiating reagent
Dehydrating reagent
Dehydrating reagent
150 ml
150 ml
150 ml
150 ml
150 ml