ALCIAN BLUE -PAS PH 2,5 -KIT MARE( L)

    SEAP
    ID
    DA1029400
    Data
    29 Iunie 2010
    Valoare
    1.869 RON
    Stare
    Oferta acceptata
    Autoritatea contractanta
    25857013 - Institutul Clinic de Boli Digestive si Transplant Hepatic Fundeni
    Localitate
    Bucuresti, Bucuresti
    Furnizor
    3573061 - S.C. TUNIC PROD S.R.L. S.R.L.
    Tipul contractului
    -
    Cod CPV
    24200000-6 - Coloranti si pigmenti (Rev.2)
    Descriere:
    ALCIAN BLUE -PAS PH 2,5 -KIT MARE( L) Alcian dyes belong to cuprophthalocyanine group; they bind polyanions of acid mucopolysaccharides through saline bounds. In this method, a sodium tetraborate solution transforms the dye into Monastral blue pigment, which is insoluble and can therefore be further manipulated without spreading in the tissue (Alcian blue-PAS). Alcian blue reacts with polyanions whose components are sulphuric and carboxylic radicals (phosphate radicals of nucleic acids do not react). As result, acid mucins only are stained. Specificity (see kit ALCIAN BLUE pH 2,5 - pH 1) - pH 1 Alcian blue ionizes strongly sulphured mucins only. - pH 2,5 Alcian blue ionizes most acid mucins. Method Bring section to distilled water. Put on the section 10 drops of reagent A: leave to act 30 minutes. Drain the slide without washing and put on the section 10 drops of reagent B : leave to act 10 minutes. Wash in distilled water. Put on the section 10 drops of reagent C: leave to act 5 minutes. Wash in distilled water. Dehydrate through ascending alcohols, clear in xylene and mount. Results Acid mucopolysaccharides Nuclei turquoise blue red Reagents Alcian Blue pH 2,5 according to Mowry Sodium tetraborate solution Carmalum according to Mayer 30 ml 30 ml 30 ml
    Achizitii
    1.869 RON
    Cantitate: 3
    Unitate masura: bucata
    ALCIAN BLUE -PAS PH 2,5 -KIT MARE( L)
    ALCIAN BLUE -PAS PH 2,5 -KIT MARE( L) Alcian dyes belong to cuprophthalocyanine group; they bind polyanions of acid mucopolysaccharides through saline bounds. In this method, a sodium tetraborate solution transforms the dye into Monastral blue pigment, which is insoluble and can therefore be further manipulated without spreading in the tissue (Alcian blue-PAS). Alcian blue reacts with polyanions whose components are sulphuric and carboxylic radicals (phosphate radicals of nucleic acids do not react). As result, acid mucins only are stained. Specificity (see kit ALCIAN BLUE pH 2,5 - pH 1) - pH 1 Alcian blue ionizes strongly sulphured mucins only. - pH 2,5 Alcian blue ionizes most acid mucins. Method Bring section to distilled water. Put on the section 10 drops of reagent A: leave to act 30 minutes. Drain the slide without washing and put on the section 10 drops of reagent B : leave to act 10 minutes. Wash in distilled water. Put on the section 10 drops of reagent C: leave to act 5 minutes. Wash in distilled water. Dehydrate through ascending alcohols, clear in xylene and mount. Results Acid mucopolysaccharides Nuclei turquoise blue red Reagents Alcian Blue pH 2,5 according to Mowry Sodium tetraborate solution Carmalum according to Mayer 30 ml 30 ml 30 ml